PD1 blockade potentiates the therapeutic efficacy of photothermally-activated and MRI-guided low temperature-sensitive magnetoliposomes

This study investigates the effect of PD1 blockade on therapeutic efficacy novel doxorubicin-loaded temperature-sensitive liposomes. Herein, we report photothermally-activated, low magnetoliposomes (mLTSL) for efficient drug delivery and magnetic resonance imaging (MRI). The mLTSL were prepared by embedding small nitrodopamine palmitate (NDPM)-coated iron oxide nanoparticles (IO NPs) in lipid bilayer liposomes (LTSL), using film hydration extrusion. Doxorubicin (DOX)-loaded characterized dynamic light scattering, differential scanning calorimetry, electron microscopy, spectrofluorimetry, atomic absorption spectroscopy. Photothermal experiments 808 nm laser irradiation conducted. In vitro photothermal DOX release studies cytotoxicity was assessed flow cytometry resazurin viability assay, respectively. vivo tumor accumulation mLTSL(DOX) fluorescence MR imaging, Finally, combination with photothermally-activated CT26-tumor model evaluated monitoring growth, cytokine immune cell infiltration tissue. Interestingly, heating obtained varying IO NPs content power, where on-demand burst achievable vivo. Moreover, our exhibited promising properties high transverse r2 relaxivity (333 mM?1 s?1), resulting superior Furthermore, potentiated anti-PD1 mAb, a significant reduction CT26 growth via activation. Our highlights potential combining mLTSL(DOX), latter could facilitate chemo/photothermal therapy MRI-guided delivery.